Naslov (srp)

Biomedical application of green synthesized nanoparticles as anti-cancer agents and nanoparticle incorporated nanofibers as potential coating for drug coated balloons: doctoral dissertation

Autor

Rehman Qamar, SafI Ur, 1996-

Doprinosi

Filipović, Nenad, 1970-
Živanović, Marko, 1980-
Milošević, Miljan, 1984-
Zdravković, Nebojša, 1969-
Cvetković, Danijela, 1982-

Opis (srp)

Green nanotechnology enables the conversion of biological systems intoenvironmentally friendly methods for synthesizing nanomaterials. Green approachesutilize biological sources as an alternative to the physicochemical creation ofnanoparticles, which involves the use of harmful chemicals and severe settings. Bycombining the principles of green chemistry, green nanotechnology, and chemicalengineering may create metal nanoparticles that are both environmentally beneficial andcost effective, without the need for harmful chemicals during their production.Therefore, in this study, we used the extract of the traditional Serbian tea Bosiljak(Ocimum basilicum L.) and Borovnica (Vaccinium myrtillus) extracts to create silvernanoparticles (AgNPs) in a single step without the use of hazardous chemicals. Thiseco-friendly, easy-to-use, and reasonably priced approach uses an aqueous plant extractas a reducing and stabilizing agent for AgNPs. The NPs synthesized from Bosiljak andBorovnica were termed as OBTe-AgNPs and VMTe-AgNPs, respectively. UV–Visspectroscopy, energy dispersive spectroscopy (EDS), transmission electron microscopy(TEM), field emission scanning electron microscopy (FESEM), and dynamic lightspectroscopy (DLS) were all used to examine the AgNPs. UV–Vis spectroscopydisplayed surface plasmon resonance (SPR) at 344 nm and 305 nm, respectively forOBTe-AgNPs and VMTe-AgNPs. The FESEM analysis of OBTe-AgNPs and VMTe-AgNPs revealed that both processes oval shape with average size of 55 and 52 nm,respectively. While and TEM analysis revealed that the average size of OBTe-AgNPsand VMTe-AgNPs was 35 nm and 80 nm, respectively. The anti-cancer ability ofOBTe-AgNPs against human cervical immortalized (HeLa) cancer cells was tested.While on the other hand, VMTe-AgNPs were used as double action tool as anti-cancerand anti-atherosclerosis against breast adenocarcinoma epithelial cells (MDA-MB-231)and human umbilical vein endothelial cells (HUVEC). The results showed that bothAgNPs possess cytotoxic effect in a dose dependent manner. OBTe-AgNPs showedIC50 value of 21.78 ± 0.68 μg/ml against HeLa cells. While VMTe-AgNPs showed IC50value of 29.69 ± 1.47 μg/ml and 17.72 ± 1.25 μg/ml against MDA-MB-231 and HUVECcells, respectively. Furthermore, the potential of OBTe-AgNPs were incorporated intonanofibers (NFs) as anti-atherosclerosis agent as well as a new coating material for drugcoated balloons (DCBs). The Ag release study reveals that there is sustainable releaseover time from various variations of OBTe-AgNPs-NF. Due to this we saw antiatherosclerosispotential of these OBTe-AgNPs-NFs when exposed to HUVEC cells. Inconclusion, AgNPs and AgNPs-NF produced via green nanotechnology have thepotential to be used as anti-cancer and anti-atherosclerosis agents. Moreover, AgNPs-NF acts as a newer coating material for DCBs to treat atherosclerosis and peripheralartery disease.

Opis (srp)

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Opis (eng)

Zelena nanotehnologija omogućava pretvaranje bioloških sistema uekološki prihvatljive metode za sintezu nanomaterijala. Zeleni pristupikoriste biološke izvore kao alternativu fizičko-hemijskom kreiranjunanočestica, što uključuje upotrebu štetnih hemikalija i teške uslove.Kombinovanjem principa zelene hemije, zelene nanotehnologije i hemijskoginženjeringa mogu se stvoriti metalne nanočestice koje su i ekološki korisnei isplative, bez potrebe za štetnim hemikalijama tokom njihove proizvodnje.Zbog toga smo u ovoj studiji koristili ekstrakt tradicionalnog srpskog čajaBosiljak (Ocimum basilicum L.) i ekstrakte Borovnice (Vaccinium myrtillus) zastvaranje nanočestica srebra (AgNPs) u jednom koraku bez upotrebe opasnihhemikalija. Ovaj ekološki prihvatljiv pristup, jednostavan za upotrebu ipristupačne cene, koristi vodeni biljni ekstrakt kao sredstvo za redukciju istabilizaciju nanočestica srebra. Nano čestice sintetisane iz Bosiljaka iBorovnice nazvani su kao OBTe-AgNPs i VMTe-AgNPs, respektivno. UV-Visspektroskopija, spektroskopija disperzije energije (EDS), transmisionaelektronska mikroskopija (TEM), emisiona skenirajuća elektronskamikroskopija (FESEM) i dinamička svetlosna spektroskopija (DLS) sukorišćeni za ispitivanje AgNPs. UV-Vis spektroskopija je pokazalapovršinsku plazmonsku rezonancu (SPR) na 344 nm i 305 nm, respektivno zaOBTe-AgNPs i VMTe-AgNPs. FESEM analiza OBTe-AgNPs i VMTe-AgNPsotkrila je da oba procesa ovalnog oblika sa prosečnom veličinom od 55 i 52 nm.Dok i TEM analiza je otkrila da je prosečna veličina OBTe-AgNPs i VMTe-AgNPs bila 35 nm i 80 nm, respektivno. Testirana je efikasnost OBTe-AgNPsu odnosu na ćelije kancera i ljudskih imortalizovanih ćelija kancera grlićamaterice (HeLa). Dok su, s druge strane, VMTe-AgNPs korišćene kao sredstvodvostrukog dejstva kao anti-kancer i anti-ateroskleroza protiv epitelnihćelija adenokarcinoma dojke (MDA-MB-231) i endotelnih ćelija humanepupčane vene (HUVEC). Rezultati su pokazali da obe AgNPs posedujucitotoksični efekat u zavisnosti od doze. OBTe-AgNPs su pokazali IC50vrednost od 21,78 ± 0,68 μg/ml prema HeLa ćelijama. Dok su VMTe-AgNPspokazali IC50 vrednost od 29,69 ± 1,47 μg/ml i 17,72 ± 1,25 μg/ml u odnosu naMDA-MB-231 i HUVEC ćelije, respektivno. Štaviše, potencijal OBTe-AgNPsje ugrađen u nanovlakna (NF) kao agens protiv ateroskleroze, kao i novimaterijal za oblaganje balona obloženih lekovima (DCB). Studija oslobađanjaAg otkriva da postoji održivo oslobađanje tokom vremena iz različitihvarijacija OBTe-AgNPs-NFs. Zbog toga smo videli antiateroskleroznipotencijal ovih OBTe-AgNPs-NF kada su bili izloženi HUVEC ćelijama. Uzaključku, AgNPs i AgNPs-NFs proizvedeni preko zelene nanotehnologije imajupotencijal da se koriste kao agensi protiv raka i ateroskleroze. Štaviše,AgNPs-NFs deluju kao noviji materijal za oblaganje za DCB za lečenjeateroskleroze i bolesti perifernih arterija.

Jezik

srpski

Datum

2024

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY 3.0 AT - Creative Commons Autorstvo 3.0 Austria License.

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