Title (srp)

Povezanost oksidacionog stresa i periapikalnih lezija kod hipertenzivnih pacova

Author

Milojević, Andjela, 1986-

Contributor

Živković, Vladimir, 1984-
Vasović, Miroslav, 1982-
Jakovljević, Vladimir, 1971-
Milosavljević, Marko, 1980-
Mališ, Miloš, 1966-

Description (srp)

Cilj ove studije je bio da ispita povezanost periapikalnih lezija, funkcije srca ioksidacionog stresa kod pacova u uslovima hipertenzije i normotenzije.Istraživanje je obuhvatilo 48 pacova Wistar soja, svrstanih u 4 eksperimentalnegrupe: I-kontrola, II-normotenzivni pacovi sa prisustvom periapikalnih lezija, IIIspontanohipertenzivni pacovi bez prisustva periapikalnih lezija, IVspontanohipertenzivni pacovi sa prisustvom periapikalnih lezija. Uzorci tkivahemimandibula korišćeni su za patohistološku, histomorfometrijsku analizu iisptivanje koncentracije proinflamatornih citokina. Određivani su markerisistemskog i srčanog oksidacionog statusa. Icpitivan je uticaj lezija na promenufunkcije izolovanog srca pacova metodom retrogradne perfuzije po Langendorff-u. Sapatohistološkog i histomorfometrijskog aspekta, debljina periodontalnogligamenta, resorpcija alveolarne kosti i apikalnog cementa, intezitet iekstenzivnost periapikalnog inflamatornog infiltrata bila je značajno veća u grupihipertenzivnih pacova. Koncentracija većine proinflamatornih citokina lezijabila je viša u hipertenzivnim stanjima. Periapikalne lezije bile su povezane saporemećenom kardiodinamikom i patohistološkim promenama izolovanih srcapacova u hipertenzivnim uslovima. Takođe, bile su u korelaciji sa povećanimvrednostima prooksidanasa i manjom mobilnošću antioksidanasa posebno uhipertenzivnom stanju. Uopšeno posmatrano, periapikalne lezije i hipertenzijameđusobno su povezane na ispitivanom modelu pacova. Sadašnji rezultati mogupomoći boljem razumevanju povezanosti periapikalnih lezija i srčane funkcije uhipertenzivnim uslovima, podržavajući pretpostavku da lezije mogu negativnouticati na funkciju srca i potencijalno poremetiti homeostazu oksidacionog statusau eksperimentalnom modelu pacova.

Description (srp)

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Description (eng)

The aim of this study was to investigate the link between periapical lesion, cardiac functionand oxidative stress in rats under conditions of hypertension and normotension. The studyincluded 48 Wistar rats, divided into 4 experimental groups: I-control, II-normotensive ratswith periapical lesions, III-spontaneously hypertensive rats without periapical lesions, IVspontaneously hypertensive rats with periapical lesions. Hemimandibular tissue samples wereused for histopathological, histomorphometric analysis and examination of the concentrationof proinflammatory cytokines. Markers of systemic and cardiac oxidative status weredetermined. The influence of lesions on the change of function of isolated rat hearts by themethod of retrograde perfusion according to the Langendorff technique was examined. Fromthe pathohistological and histomorphometric aspects, periodontal ligament thickness, alveolarbone and apical cementum resorption, intensity and extension of inflammatory infiltrate weresignificantly higher in the hypertensive rats. The concentration of most proinflammatorycytokines in lesions was significantly higher in hypertensive conditions. Periapical lesionswere associated with impaired cardiodynamics and pathohistological changes on isolated rathearts in hypertensive conditions. Also, they were correlated with increased values ofprooxidants and decreased mobility of antioxidants in a hypertensive state. In general,periapical lesions and hypertension were correlated in a rat model. Results may help to betterunderstand the association between lesions and cardiac function in hypertensive conditions,supporting the hypothesis that lesions may adversely affect cardiac function and potentiallydisrupt oxidative status homeostasis in an experimental rat model.

Object languages

Serbian

Date

2022

Rights

Creative Commons License
This work is licensed under a
CC BY-NC-ND 3.0 AT - Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Austria License.

http://creativecommons.org/licenses/by-nc-nd/3.0/at/legalcode

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