Title (srp)

Prekondicioniranje ishemijsko reperfuzionepovrede srca pacova različitim tipovimatrenažnih procesa

Author

Glišić, Miloš, 1992-

Contributor

Živković, Vladimir, 1984-
Jakovljević, Vladimir, 1971-
Srejović, Ivan, 1985-
Radovanović, Dragan, 1975-

Description (srp)

Podaci o uticaju različitih tipova trenažnih aktivnosti na funkcionalnioporavak srca nakon ishemijsko/reperfuzionog oštećenja su još uvek jakoneusaglašeni. Dodatni problem prestavlja činjenica, da i dalje nije poznato koji tiptreninga i njegovog intenziteta (aerobni ili anaerobni) ostvaruje najbolje rezultateu prekondicioniranju miokarda. Cilj ove studije je ispitivanje efekataprekondicioniranja vežbanjem na animalnom modelu ishemijsko/reperfuzionepovrede (I / R) miokarda pacova odnosno utvrđivanje uticaja različitih tipovatrenažnih procesa na I / R, kao i uloge oksidacionog stresa u dobijenim efektima.Studija je sprovedena na 40 pacova razvrstanih u jednu kontrolnu (sedenterni pacovi)i četiri eksperimentalne grupe. Pacovi iz eksperimentalnih grupa su bilipodvrgnuti trenažnom protokolu prekondicionoranja trčanjem i plivanjem (uaerobnoj i anaerobnoj formi). Nakon trenažnih protkola životinje su žrtvovane, aizolovana srca perfundovana metodom retrogradne perfuzije po Langendorff-u prikonstantnom koronarnom perfuzionom pritisku od 70 smH2O. Nakon stabilizacije,srca su bila podvrgnuta globalnoj ishemiji u trajanju od 20 minuta, a potom ireperfuziji u trajanju od 30 minuta. Vrednosti koronarnog protoka su bileodređivane floumetrijski, a parametri funkcije leve komore (dp/dt max, dp/dt min,SLVP, DLVP i HR) su kontinuirano praćeni. U uzorcima koronarnog venskogefluenta, kao i u krvi pacova spektrofotometrijskim metodama su određivaniparametri oksidacionog stresa. Standardnom histološkom analizom su utvrđivanepromene na srcu i kvadricepsu. Obe vrste treninga samo pri aerobnim intenzitetimadovele su do efikasnijeg oporavka većine kardiodinamskih pokazatelja, dok uanaerobnim zonama nisu uspele da ostvare bolje efekte u odnosu na sedaterne pacove.Osim toga, obe vrste treninga bile su povezane sa povećanom produkcijom srčanihpro-oksidanasa, pri čemu je anaerobni intenzitet doveo do najvećeg porasta njihoveprodukcije.

Description (srp)

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Description (eng)

Data on the effects of different types of training activities on the functional recovery of theheart after ischemia/reperfusion damage are still very inconsistent. An additional problem isthe fact that it is still not known which type of training and its intensity (aerobic or anaerobic)achieves the best results in myocardial preconditioning. The aim of this stidy wasexamination of the effects of exercise preconditioning on an animal model ofischemia/reperfusion injury (I/R) of the rat myocardium, i.e. determination of the influence ofdifferent types of training processes on I/R, as well as the role of oxidative stress in theobtained effects. The study was conducted on 40 rats classified into one control (sedentaryrats) and four experimental groups. Rats from the experimental groups were subjected to atraining protocol of preconditioning by running and swimming (in aerobic and anaerobicform). After the training protocols, the animals were sacrificed, and the isolated hearts wereperfused using the Langendorff retrograde perfusion method at a constant coronary perfusionpressure of 70 cmH2O. After stabilization, hearts were subjected to global ischemia for 20min and then reperfusion for 30 min. Coronary flow values were determined flowmetrically,and left ventricular function parameters (dp/dt max, dp/dt min, SLVP, DLVP and HR) werecontinuously monitored. Oxidative stress parameters were determined in samples of coronaryvenous effluent, as well as in the blood of rats using spectrophotometric methods. Changes inthe heart and quadriceps were determined by standard histological analysis. Both types oftraining only at aerobic intensities led to more efficient recovery of most cardiodynamicparameters, while in anaerobic zones they failed to achieve better effects compared tosedentary rats. In addition, both types of training were associated with increased productionof cardiac pro-oxidants, with anaerobic intensity leading to the greatest increase in theirproduction.

Object languages

Serbian

Date

2024

Rights

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CC BY-NC-ND 3.0 AT - Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Austria License.

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