Title (srp)

Efekti ekstrakata biljne vrste Viscum album L. na toksičnost insekticida hlorpirifosa i imidakloprida kod različitih eksperimentalnih model sistema

Author

Milošević, Marija D.,

Contributor

Ognjanović, Branka, 1962-
Mašković, Pavle, 1983-
Matić, Miloš, 1984-
Gavrić, Jelena

Description (srp)

Hlorpirifos (CPF) i imidakloprid (IMI) su insekticidi koji se široko upotrebljavaju u zaštitibiljaka. Viscum album L. je semiparazitska biljka koja ima tradicionalnu upotrebu u lečenjubrojnih bolesti. Ciljevi ove doktorske disertacije su ispitivanje ekstrakata listova imele (VAE)na toksičnost izazvanu CPF i IMI u in vitro i in vivo uslovima. Dobijeni rezultati pokazuju daCPF i IMI ispoljavaju citotoksične efekte, dok kotretmani sa VAE dovode do povećanjavijabilnosti ćelija. CPF smanjuje RBC i Hb, dok IMI smanjuje broj PLT u odnosu na kontrolu.CPF i IMI dovode do povećanja ALT, AST, LDH i bilirubina. Holesterol i trigliceridi supovećani kod CPF i IMI grupe, a koncentracija glukoze je povećana u CPF grupi u poređenjusa kontrolom. Aktivnost AChE je smanjena u jetri CPF i IMI grupe. Povišena koncentracijaLPO je konstatovana u jetri i testisu nakon tretmana CPF, i u jetri u IMI grupi. KoncentracijaGSH u CPF i IMI grupi su smanjene u jetri, a koncentracija vitamina C je povećana u jetri CPFgrupe i jetri i testisu IMI grupe u poređenju sa kontrolom. CPF i IMI dovode i do promenaaktivnosti SOD, CAT, GSH-Px, GR i GST, i izazivaju patomorfološke promene jetre i bubrega.VAE ublažava hematotoksične efekte, smanjuje aktivnosti ALT i AST, koncentraciju glukoze,ukupnog holesterola i triglicerida i koncentraciju LPO. Ispitivani ekstrakt dovodi dostabilizacije promena aktivnosti SOD, CAT, GSH-Px, GR i GST, i delimično ublažavapatološke promene u jetri i bubrezima. Ova doktorska disertacija pruža detaljniji uvid u toksičnodelovanje CPF i IMI. Iz dobijenih rezultata došlo se do novih saznanja o protektivnompotencijalu VAE, i njenim mehanizmima delovanja, što predstavlja značajan doprinospostojećim naučnim podacima.

Description (srp)

-

Description (eng)

Chlorpyrifos (CPF) and imidacloprid (IMI) are insecticides widely used to protect plants.Viscum album L. is a semi-parasitic plant with a traditional use in treating numerous diseases.This doctoral dissertation aims to test mistletoe leaf extracts (VAE) for toxicity caused by CPFand IMI in in vitro and in vivo conditions. The results show that CPF and IMI exhibit cytotoxiceffects, while co-treatments with VAE increased cell viability. CPF decreases RBC and Hb,while IMI decreases PLT count compared to control. CPF and IMI increase ALT, AST, LDHand bilirubin. Cholesterol and triglycerides were increased in the CPF and IMI groups, andglucose concentration was raised in the CPF group compared to the control. AChE activity wasdecreased in the liver of the CPF and IMI groups. An increased concentration of LPO was notedin the liver and testis after CPF treatment, and in the liver in the IMI group. The concentrationof GSH in the CPF and IMI group was decreased in the liver, and the concentration of vitaminC was increased in the liver of the CPF group and the liver and testis of the IMI group comparedto the control. CPF and IMI also lead to changes in the activities of SOD, CAT, GSH-Px, GRand GST, and cause pathomorphological changes in the liver and kidneys. VAE alleviateshematotoxic effects, reduces ALT and AST, glucose concentration, total cholesterol andtriglycerides, and LPO. The extract stabilizes changes in the activities of SOD, CAT, GSH-Px,GR and GST, and partially alleviates the pathological changes in the liver and kidneys. Thisresearch provides a more detailed insight into the toxic effects of CPF and IMI. The obtainedresults have contributed to new knowledge about the protective potential of VAE and itsmechanisms of action, which represents a significant contribution to existing scientific data.

Object languages

Serbian

Date

2023

Rights

Creative Commons License
This work is licensed under a
CC BY 3.0 AT - Creative Commons Attribution 3.0 Austria License.

http://creativecommons.org/licenses/by/3.0/at/legalcode

Identifiers