Title (srp)

Fitohemijska karakterizacija ekstrakata biljaka Lythrum salicaria L. i Salvia Pratensis L., njihova biološka aktivnost i potencijalna primena u sintezi nanočestica srebra

Author

Srećković, Nikola, 1993-

Contributor

Mihailović, Vladimir, 1984-
Mišić, Danijela, 1975-
Kreft, Samo, 1972-
Beškoski, Vladimir, 1975-
Vuković, Nenad, 1976-
Matić, Miloš, 1984-

Description (srp)

U okviru istraživanja koja su obuhvaćena ovom doktorskom disertacijom određen je fenolni profil i neki aspekti farmakološke primene metanolnihekstrakata nadzemnih delova i korena biljaka Lythrum salicaria L. i Salvia pratensis L., koje imaju upotrebu u narodnoj medicini. Utvrđeno je da obe biljne vrste poseduju visoke koncentracije fenolnih jedinjenja, naročito S. pratensis koja je bogataruzmarinskom kiselinom i njenim derivatima. Identifikovana fenolna jedinjenja u ekstraktima pokazala su visok stepen biodostupnosti tokom procesa in vitrogastrointestinalne digestije. U skladu sa visokim sadržajem fenolnih jedinjenja, ekstrakti su pokazali izražen antioksidativni efekat i mogućnost zaštite DNK od oksidativnog oštećenja u in vitro uslovima. Ekstrakti obe biljke ispoljili su slabu do umerenu antimikrobnu aktivnost, kao i biokompatibilnost sa zdravim humanim keratinocitima kože (HaCaT) u koncentracijama do 100 µg/mL, dok su ekstrakti korena obe biljke pokazali citotoksičan efekat prema kancerogenim ćelijama.Uzimajući u obzir da su L. salicaria i S. pratensis posedovale visok sadržaj fenolnih jedinjenja i izraženu antioksidatvinu aktivnost, njihovi vodeni ekstrakti iskorišćeni su u zelenoj sintezi nanočestica srebra (AgNP). Dobijene AgNP su pokazale visok stepen antibakterijske aktivnosi, dok je antioksidativna aktivnost sintetisanih AgNP niža u poređenju sa odgovarajućim ekstrakatima. Nanočestice nisu pokazale hemolitičku aktivnost u koncentracijama do 150 µg/mL, dok nanočestice S.pratensis nisu ispoljile ni citotoksični efekat (do 200 µg/mL) prema ćelijama kancera i normalnim ćelijama. L. salicaria AgNP bile su efikasne u smanjenju rasta kancerogenih ćelija (IC50 od 12,7 do 62 µg/mL). Obe vrste AgNP pokazale su se kao efikasni katalizatori u reakcijama redukcije polutanata.

Description (srp)

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Description (eng)

The phenolic profile and some aspects of the pharmacological application of methanolicextracts of Lythrum salicaria L. and Salvia pratensis L. aerial parts and roots, plants used in folk medicine, were determined within the scope of the research included in this PhD thesis. It was found that both plant species have high concentrations of phenolic compounds, especially S. pratensis, which is rich in rosmarinic acid and its derivatives. The identified phenolic compounds in the extracts showed a high degree of bioavailability during the process of in vitrogastrointestinal digestion. In accordance with the high content of phenolic compounds, the extracts showed a pronounced antioxidant effect and the ability to protect DNA from oxidative damage under in vitro conditions. Extracts of both plants showed weak to moderate antimicrobial activity, as well as biocompatibility with healthy human skin keratinocytes (HaCaT) in concentrations up to 100 µg/mL, while root extracts of both plants showed a cytotoxic effect against cancerous cells.Considering that L. salicaria and S. pratensis possessed a high content of phenolic compounds and pronounced antioxidant activity, their aqueous extracts were used for the green synthesis of silver nanoparticles (AgNP). The obtained AgNPs showed a high antibacterial activity, while the antioxidant activity of the synthesized AgNPs was lower compared to the corresponding extracts. Nanoparticles did not show hemolytic activity in concentrations up to 150 µg/mL, and S. pratensis nanoparticles did not show a cytotoxic effect (up to 200 µg/mL) towards cancer cells and normal cells. L. salicaria AgNPs were effective in reducing the growth of cancer cells (IC50 from 12.7 to 62 µg/mL). Both types of AgNPs proved to be effective catalysts in reactions of pollutants reduction

Object languages

Serbian

Date

2024

Rights

Creative Commons License
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CC BY-NC-SA 3.0 AT - Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Austria License.

http://creativecommons.org/licenses/by-nc-sa/3.0/at/legalcode

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