Title (srp)

Terapijski efekti eritropoetina u direktnom prekrivanju inflamirane zubne pulpe

Author

Papić, Miloš, 1992-

Contributor

Popović, Milica, 1965-
Ljujić, Biljana, 1974-
Tanasković, Irena, 1973-
Radović, Ivana, 1976-

Description (eng)

Introduction Direct pulp capping presents placing of certain materials on exposure site inorder to preserve its vitality. The success rate of this therapy is significantly reduced if it isperformed on previously inflamed dental pulp. Erythropoietin (EPO) is the main stimulator oferythropoiesis, but the existing literature indicates that it could have various beneficial effectson repair of damaged tissues.Aim To investigate the expression of erythropoietin and erythropoietin receptor in pulpexposure and direct pulp capping and to investigate the effects of erythropoietin oninflammation and reparative dentinogenesis in direct capping of inflamed dental pulp.Material and methods Pulp inflammation was induced by exposing the pulp of rats’maxillary first molars for 48 h, followed by capping using rhEPO, Ca(OH)2, MTA orMTA+rhEPO. Noncapped exposed pulp (EC) and healthy pulp (HC) were used as controls.Immunohistochemical, histological, PCR and spectrophotometric analysis were performed.Acquired data was analyzed statistically.Results EC group had the highest expression of EPO-R, while capping with Ca(OH)2 orMTA led to increased EPO expression. Direct pulp capping decreased the expression of proinflammatory and increased the expression of immunoregulatory cytokines compared to ECgroup. Capping with MTA+rhEPO led to increased expression of odontoblast activitymarkers compared to EC group. Capping with the rhEPO-containing materials reduced thevalues of pro-oxidative parameters compared to EC group.Conclusion EPO and EPO-R are expressed in exposed and capped dental pulp. EPO can besuitable for direct pulp capping because it reduces the degree of inflammation and stimulatesthe reparative dentinogenesis, having similar effects to commonly used materials.

Description (srp)

Uvod Direktno prekrivanje predstavlja postavljanje određenih materijala na mestoeksponiranja pulpe kako bi se održao njen vitalitet. Stopa uspeha terapije značajnose umanjuje ako se sprovodi na prethodno inflamiranoj zubnoj pulpi. Eritropoetin(EPO) je glavni stimulator eritropoeze, ali postojeća saznanja ukazuju da možeostvariti različite povoljne efekte u reparaciji oštećenih tkiva.Cilj Ispitivanje ekspresije eritropoetina i eritropoetinskog receptora ueksponiranoj i direktno prekrivenoj pulpi i ispitivanje efekata eritropoetina nainflamaciju i reparatornu dentinogenezu u terapiji direktnim prekrivanjeminflamirane zubne pulpe.Materijal i metode Indukovana je inflamacija eksponiranjem pulpe prvihmaksilarnih molara pacova na 48 sati, a zatim direktno prekrivanje primenom rhEPO,Ca(OH)2, MTA ili MTA+rhEPO. Kao kontrole korišćene su eksponirananeprekrivena (EC) i zdrava zubna pulpa (HC). Sprovedene su imunohistohemijska,histološka, PCR i spektrofotometrijska analiza. Dobijeni podaci su obrađenistatistički.Rezultati EC grupa imala je najvišu ekspresiju EPO-R, dok je prekrivanje primenomCa(OH)2 ili MTA dovelo do povećanja ekspresije EPO. Direktno prekrivanje umanjiloje ekspresiju proinflamatornih citokina i povećalo ekspresiju imunoregulatornihcitokina u odnosu na EC grupu. Primena MTA+rhEPO dovela je do povećanjaekspresije markera odontoblaste aktivnosti u odnosu na EC grupu. Primenapreparata koji sadrže rhEPO umanjila je vrednosti prooksidativnih parametara uodnosu na EC grupu.Zaključak ERO i ERO-R eksprimirani su u eksponiranoj i direktno prekrivenojzubnoj pulpi. EPO može biti pogodan za primenu u terapiji direktnim prekrivanjempulpe jer umanjuje stepen inflamacije i stimuliše reparatornu dentinogenezu,ostvarujući efekat sličan uobičajeno korišćenim materijalima.

Description (srp)

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Object languages

Serbian

Date

2022

Rights

Creative Commons License
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