Naslov (srp)

Značaj antioksidantne suplementacije N-acetilcisteinom u tretmanu manifestacija neurotoksičnosti izazvane cisplatinom kod pacova

Autor

Vuković, Rade, 1977-

Doprinosi

Selaković, Dragica, 1984-
Rosić, Gvozden, 1964-
Jakovljević, Vladimir, 1971-
Ilić, Tihomir, 1966-

Opis (srp)

Uvod: Studija razmatra uticaj antioksidativnog efekta N-acetilcisteina(NAC) neurotoksične manifestacije izazvane upotrebom cisplatine kod pacova.Materijal i metode: Studija je obuhvatila 32 pacova Wistar albino pacova,starosti 2 meseca, telesne mase od 200 do 250 g, podeljenih u 4 grupe: kontrolna,cisplatinska, cisplatina + NAC grupa i NAC grupa. Cisplatina je primenjivana upojedinačnoj dozi (7.5 mg/kg) petog dana dok je antioksidantna suplementacija Nacetilcisteinom primenjivan u odgovarajućim grupama prvog i petog dana (500mg/kg). Promene u ponašanju su se utvrđivale desetog dana pomoću bihevioralnihtestova ( test otvorenog polja, test uzdignutog krstatstog lavirinta i test kačenja orep). Nakon ovih testiranja životinje su bile žrtvovane i tkivo hipokampusa iprefrontalnog korteksa je analizirano radi određivanja markera oksidativnog stresai apoptoze.Rezultati: Rezultati bihevioralnog ispitivanja su pokazali anksiogeniefekat cisplatine. Bihevioralni testovi su takođe ukazali na antidepresantniefekat cisplatine što nije u skladu sa očekivanjima na osnovu rezultata izdosadašnjih studija, pa se taj zalključak ne može sa sigurnošću izvesti i potrebno sudodatne studije i analize. Takođe, smanjenje neurotoksičnosti izazvane cisplatinom,u smislu uticaja na vrednosti parametara oksidativnog stresa i apoptoze je ostvarenoprimenom N-acetilcisteina i u tkivu hipokampusa i u tkivu prefrontalnog korteksapacova.Zaključak: Anksiogeni efekat, kao manifestacija neurotoksičnosti izazvanecisplatinom, je u bihevioralnim testovima bio uspešno umanjen oralnom primenomN-acetilcisteina.

Opis (srp)

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Opis (eng)

Introduction: A review study affects the antioxidant effect of Nacetylcysteine (NAC) neurotoxic manifestations and the use of cisplatin in rats.Material and methods: The study included 32 Wistar albino rats, 2 months old, bodyweight 200 to 250 g, divided into 4 groups: control, cislinate, target + NAC group and NACgroup. Target administration was in a single dose (7.5 mg/ kg) on the fifth day whileantioxidant supplementation with N-acetylcysteine was administered in the approval groupson the first and fifth day (500 mg/kg). Behavioral changes were determined on the tenth dayusing behavioral tests (open field test, elevated cross maze test, and tail climbing test). Afterthese tests, the animals were sacrificed and the tissue of the hippocampus and prefrontalcortex was analyzed to determine the marketing of oxidative stress and apoptosis.Results: The results of the behavioral study showed an anxiogenic effect of cislite.Behavioral tests also indicated an antidepressant effect of cisplatina, which is not in line withexpectations based on the results from previous studies and this conclusion cannot bereported with certainty and additional studies and analyzes are needed. Also, the reduction ofacid-induced neurotoxicity, in terms of the influence on the values of oxidative stress andapoptosis parameters, is created by appropriate N-acetylcysteine both in the hippocampaltissue and in the tic of the rat prefrontal cortex.Conclusion: The anxiogenic effect, as a manifestation of neurotoxicity and the use ofcislinoma, was successfully reduced in behavioral tests by oral administration of Nacetylcysteine.

Jezik

srpski

Datum

2021

Licenca

Ovo delo je licencirano pod uslovima licence
Creative Commons CC BY-NC-ND 3.0 AT - Creative Commons Autorstvo - Nekomercijalno - Bez prerada 3.0 Austria License.

http://creativecommons.org/licenses/by-nc-nd/3.0/at/legalcode